Supplementary MaterialsS1 Fig: Effects of DTx treatment about splenic DCs and MZ macrophages. (1.5M) GUID:?4CB27CDE-60FB-4F3A-8EF6-8671994857BF S2 Fig: Effects of MZ macrophage depletion about acute malaria. (A-D) B6 mice were treated with either a low dose of ClLip to deplete MARCO+ and MOMA-1+ macrophages or with PBSLip as settings. The mice were i.p. infected with 1 106 iRBCs 24 h later on. (A) Representative contour plots acquired 24 h after treatment by circulation cytometry confirm the effectiveness of ClLip-induced depletion of MARCO+ and MOMA-1+ cells without influencing CD11c+I-A+ and F4/80+ cells. Data display the percentages of MARCO+, MOMA-1+, CD11c+I-A+ and F4/80+ cells in the splenocyte populace. (B) Parasitemia curves are shown (means SD). (C) Survival curves are demonstrated. (D) Data display the percentages of proliferating CFSElowCD4+ T cells and IFN- concentrations in the supernatants of spleen cell cultures stimulated for 72 h with iRBCs (means SD). In A-D, one representative experiment out of three (n = 5) is definitely demonstrated.(PDF) ppat.1004598.s002.pdf (1.6M) GUID:?AD2D6D83-6FF9-4EA6-A2AA-80F87E1A9981 S3 Fig: Effects of DC depletion within the blood stages of infection with iRBCs or sporozoites. (A-C) B6 and B6.CD11c-DTR mice were treated with either DTx to deplete CD11c+ cells or PBS like a control. The mice were i.p. infected with 1 106 iRBCs 24 h later on. (A) Parasitemia curves are demonstrated (means SD). (B) Variations in body weight relative to day time 0 are shown (means TAS-115 SD). (C) Survival curves are demonstrated. (D-F) B6 and B6.CD11c-DTR mice were i.v. infected with 1 103 sporozoites. After 48 h, the mice were treated with DTx to deplete CD11c+ cells at the beginning of blood stage. (D) Parasitemia curves are demonstrated (means SEM). (E) Variations in body weight relative to day time 0 are demonstrated (means SEM). (F) Survival curves are demonstrated. In A-F, significant variations (p 0.05) between the indicated organizations are designated by *. In A-C, one representative experiment out of three (n = 3-4) is definitely demonstrated. In D-F, data from three experiments (n = 2-3) are demonstrated.(PDF) ppat.1004598.s003.pdf (905K) GUID:?6FAA56EB-50E4-4C53-B7EB-F809AF8DCD8C S4 Fig: Phagocytosis of iRBCs by splenic DC subsets throughout acute malaria. Spleens were analyzed 15 min after i.v. injection of 1 1 108 adult CTV-iRBCs (dark collection histograms) or PBS (packed histograms) in B6 mice at zero, five or eight days p.i. with 1 106 iRBCs. (A) Representative histograms acquired by circulation cytometry display CTV staining in the splenic DC subsets (CD11b+, CD8+, B220+ or CD4+). Data display the percentages of CTV+ cells in each subset. (B) Numbers of total and CTV+CD11c+ cells per spleen were calculated from the data obtained inside a. In B, significant variations (p 0.05) between the DC subsets at different days p.i. are designated by *. INSIDE A and B, one representative experiment out of three (n = 5) is definitely demonstrated.(PDF) ppat.1004598.s004.pdf (1.0M) GUID:?4668EAFA-EFD5-428D-8ACA-3058046DBF73 S5 Fig: Phenotypic analysis of YFP+ cells soon after infection and during pre-crisis. (A MEKK13 and B) Spleens were analyzed 15 min after i.v. injection of 1 1 108 adult CTV-iRBCs in B6.CD11c-YFP mice at zero or five days p.i. with 1 106 iRBCs. (A) Representative contour plots display the gate strategy for analysis of YFP+ cells in na?ve mice. Data display the percentages of singlets, leukocytes and YFP+ cells in each contour storyline. TAS-115 (B) Representative contour plots display CD11c and F4/80 staining in the YFP+ cells. Data display the percentages TAS-115 of these cells in the YFP+ cell populace. Histograms display MHC class II (I-A) staining in CD11c+YFP+ cells. The fluorescence minus one (FMO) control was acquired in CD11c+YFP+ cells from a [(0) + CTV-malaria. B6 mice were i.p. infected with 1 106 iRBCs. At zero, five or eight days p.i., spleens were analyzed by circulation cytometry. (A) Representative histograms display the manifestation of CD36 and FcRI in CD11c+ cells. The related FMO control for each marker is displayed by the packed histograms. (B) Median fluorescence intensity (MFI) was determined from the data obtained inside a (means TAS-115 SD). (C) Representative histograms display the manifestation of MHC class II (I-A), CD80 and CD86 molecules in CD11c+ cells. The related FMO control for each marker is displayed by the packed histograms..