Three-dimensional animation from the huge LCV shown in S8f Fig using the edges from the binary mask useful for volume analysis highlighted in green

Three-dimensional animation from the huge LCV shown in S8f Fig using the edges from the binary mask useful for volume analysis highlighted in green.(AVI) ppat.1006088.s009.avi (120K) GUID:?82A16217-AE69-455C-B699-92B9DDEEF9A7 Data Availability StatementAll relevant data are inside the paper and its own Supporting Information documents. Abstract Vacuolar bacterial pathogens are sheltered within exclusive membrane-bound organelles that expand as… Continue reading Three-dimensional animation from the huge LCV shown in S8f Fig using the edges from the binary mask useful for volume analysis highlighted in green

Due to the different geometry, the system shook on a Plate (Orbit 3 mm) at 150 rpm , while the Petri dishes were shaken on an orbital (orbit 20 mm) at 50 rpm

Due to the different geometry, the system shook on a Plate (Orbit 3 mm) at 150 rpm , while the Petri dishes were shaken on an orbital (orbit 20 mm) at 50 rpm. found more cell viability in smaller diameter spheroids than larger ones by using the apoptosis test. Furthermore, there is no positive influence… Continue reading Due to the different geometry, the system shook on a Plate (Orbit 3 mm) at 150 rpm , while the Petri dishes were shaken on an orbital (orbit 20 mm) at 50 rpm

Supplementary MaterialsSupplementary Information 42003_2019_514_MOESM1_ESM

Supplementary MaterialsSupplementary Information 42003_2019_514_MOESM1_ESM. measurements of endogenous Piezo1 Roburic acid activity and grip forces in native cellular conditions, we show that cellular traction forces generate spatially-restricted Piezo1-mediated Ca2+ flickers in the absence of externally-applied mechanical forces. Although Piezo1 channels diffuse readily in the plasma membrane and are widely distributed across the cell, their flicker activity… Continue reading Supplementary MaterialsSupplementary Information 42003_2019_514_MOESM1_ESM

Supplementary MaterialsAdditional file 1

Supplementary MaterialsAdditional file 1. or 2-deoxyglucose, failed to discriminate between commercial wine candida strains with different nutritional requirements, but evidenced genetic variability among industrial isolates, and between laboratory and commercial strains. Most signaling pathways involve events of protein phosphorylation that can be adopted as markers of their activity. The main pathway to promote growth in… Continue reading Supplementary MaterialsAdditional file 1