Ubiquitin receptors connect substrate ubiquitylation to proteasomal degradation. S-sepharose resin respectively. Each resin was permitted to mix at 4C overnight with K48- or K63-linked tetraubiquitin (Boston Biochem Inc.), or octaubiquitin, and then washed extensively with buffer A (20 mM sodium phosphate (pH 6.5), 50 mM NaCl, 0.5% (v/v) Triton X-100). The resin was next incubated… Continue reading Ubiquitin receptors connect substrate ubiquitylation to proteasomal degradation. S-sepharose resin respectively.