The real-time quantitative polymerase chain reaction (rtqPCR) has overcome the limitations of conventional, time-consuming quantitative PCR strategies and is maturing into a routine tool to quantify gene expression levels, following reverse transcription (RT) of mRNA into complementary DNA (cDNA). expression of individual cells and thus could complement global microarray-based expression profiling strategies. INTRODUCTION Gene expression… Continue reading The real-time quantitative polymerase chain reaction (rtqPCR) has overcome the limitations